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Registros recuperados: 34
Primeira ... 12 ... Última
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Chilean IPNV isolates: Robustness analysis of PCR detection Electron. J. Biotechnol.
Jorquera,Esteban; Morales,Paz; Tapia,David; Torres,Pamela; Eissler,Yoanna; Espinoza,Juan C; Conejeros,Pablo; Kuznar,Juan.
Background: The genomes of several infectious pancreatic necrosis viruses (IPNVs) isolated in Chile were sequenced with a single amplification approach for both segments A and B. The resulting sequences were then used to determine the conservation of the primer-binding regions used in polymerase chain reaction (PCR)-based diagnostic methods proposed in the literature. Thus, the robustness of each technique was studied, particularly the eventual effect of further mutations within the primer-binding sites. Results: On analysis, most methods currently used to detect Chilean IPNV varieties were deemed adequate. However, the primers were designed to be genogroup specific, implying that most detection methods pose some risk of detecting all strains prevalent in...
Tipo: Journal article Palavras-chave: IPNV detection; Mismatch's Tm analysis; QPCR.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200005
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Expression analysis in response to drought stress in soybean: shedding light on the regulation of metabolic pathway genes Genet. Mol. Biol.
Guimarães-Dias,Fábia; Neves-Borges,Anna Cristina; Viana,Antonio Americo Barbosa; Mesquita,Rosilene Oliveira; Romano,Eduardo; Grossi-de-Sá,Maria de Fátima; Nepomuceno,Alexandre Lima; Loureiro,Marcelo Ehlers; Alves-Ferreira,Márcio.
Metabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Drought resistance; QPCR; Metabolic pathway; Bioinformatics.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200004
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Ciguatoxin Occurrence in Food-Web Components of a Cuban Coral Reef Ecosystem: Risk-Assessment Implications ArchiMer
Díaz-asencio, Lisbet; Clausing, Rachel J.; Vandersea, Mark; Chamero-lago, Donaida; Gómez-batista, Miguel; Hernández-albernas, Joan I.; Chomérat, Nicolas; Rojas-abrahantes, Gabriel; Litaker, Wayne; Tester, Patricia; Diogène, Jorge; Alonso-hernández, Carlos M.; Dechraoui Bottein, Marie-yasmine.
In Cuba, ciguatera poisoning associated with fish consumption is the most commonly occurring non-bacterial seafood-borne illness. Risk management through fish market regulation has existed in Cuba for decades and consists of bans on selected species above a certain weight; however, the actual occurrence of ciguatoxins (CTXs) in seafood has never been verified. From this food safety risk management perspective, a study site locally known to be at risk for ciguatera was selected. Analysis of the epiphytic dinoflagellate community identified the microalga Gambierdiscus. Gambierdiscus species included six of the seven species known to be present in Cuba (G. caribaeus, G. belizeanus, G. carpenteri, G. carolinianus, G. silvae, and F. ruetzleri). CTX-like...
Tipo: Text Palavras-chave: Caribbean; Ciguatoxicity; QPCR; Trophic transfer; Ish; Food safety; Food security; Science-based management; Foodborne disease.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00597/70947/69189.pdf
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Environmental DNA for detecting Bulinus truncatus : A new environmental surveillance tool for schistosomiasis emergence risk assessment ArchiMer
Mulero, Stephen; Boissier, Jérôme; Allienne, Jean‐françois; Quilichini, Yann; Foata, Joséphine; Pointier, Jean‐pierre; Rey, Olivier.
Under ongoing climate changes, the development of large‐scale monitoring tools for assessing the risk of disease emergence constitutes an urging challenge. This is particularly the case for snail‐borne diseases such as the urogenital bilharziasis that emerged in Corsica and threat European countries. The expansion of this tropical disease mainly relies on the local presence of competent snail hosts such as Bulinus truncatus. Unfortunately, very little is known about the actual repartition of freshwater snails worldwide which makes new emergences difficult to predict. In this study, we developed two ready‐to‐use environmental DNA‐based methods for assessing the distribution of B. truncatus from water samples collected in the field. We used two approaches, a...
Tipo: Text Palavras-chave: Bulinus truncatus; Corsica; DdPCR; Environmental DNA; Environmental monitoring; QPCR; Schistosomiasis.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00593/70554/68723.pdf
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Assessment of Ciguatera and Other Phycotoxin-Related Risks in Anaho Bay (Nuku Hiva Island, French Polynesia): Molecular, Toxicological, and Chemical Analyses of Passive Samplers ArchiMer
Roué, Mélanie; Smith, Kirsty F.; Sibat, Manoella; Viallon, Jérôme; Henry, Kévin; Ung, André; Biessy, Laura; Hess, Philipp; Darius, Hélène Taiana; Chinain, Mireille.
Ciguatera poisoning is a foodborne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates from the genera Gambierdiscus and Fukuyoa. The suitability of Solid Phase Adsorption Toxin Tracking (SPATT) technology for the monitoring of dissolved CTXs in the marine environment has recently been demonstrated. To refine the use of this passive monitoring tool in ciguateric areas, the effects of deployment time and sampler format on the adsorption of CTXs by HP20 resin were assessed in Anaho Bay (Nuku Hiva Island, French Polynesia), a well-known ciguatera hotspot. Toxicity data assessed by means of the mouse neuroblastoma cell-based assay (CBA-N2a) showed that a 24 h deployment of 2.5 g of resin allowed...
Tipo: Text Palavras-chave: Ciguatera monitoring; Gambierdiscus; Ciguatoxins; SPATT passive samplers; HP20 resin; CBA-N2a; LC-MS; MS; WS artificial substrate; QPCR; HTS metabarcoding.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00629/74143/73670.pdf
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Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna ( Thunnus obesus ) and Yellowfin Tuna ( Thunnus albacares ), in Canned Tuna ArchiMer
Bojolly, Daline; Doyen, Perine; Le Fur, Bruno; Christaki, Urania; Verrez-bagnis, Veronique; Grard, Thierry.
Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one...
Tipo: Text Palavras-chave: Tuna; Authentication; Quantification; Canned products; TaqMan; QPCR; Species identification; Bigeye tuna (Thunnus obesus); Yellowfin tuna (Thunnus albacares).
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00371/48217/48403.pdf
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Differential Mortality and High Viral Load in Naive Pacific Oyster Families Exposed to OsHV-1 Suggests Tolerance Rather than Resistance to Infection ArchiMer
Agnew, M. Victoria; Friedman, Carolyn S.; Langdon, Christopher; Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Degremont, Lionel; Dhar, Arun K.; Kirkland, Peter; Dumbauld, Brett; Burge, Colleen A..
Pacific oysters, Crassostrea gigas, are one of the most productive aquaculture species in the world. However, they are threatened by the spread of Ostreid herpesvirus-1 (OsHV-1) and its microvariants (collectively “µvars”), which cause mass mortalities in all life stages of Pacific oysters globally. Breeding programs have been successful in reducing mortality due to OsHV-1 variants following viral outbreaks; however, an OsHV-1-resistant oyster line does not yet exist in the United States (US), and it is unknown how OsHV-1 µvars will affect US oyster populations compared to the current variant, which is similar to the OsHV-1 reference, found in Tomales Bay, CA. The goals of this study were to investigate the resistance of C. gigas juveniles produced by the...
Tipo: Text Palavras-chave: OsHV-1; Mu vars; Oyster; Virus; Tolerance; Virulence; QPCR.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00665/77739/79862.pdf
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Rearing practices identified as risk factors for ostreid herpesvirus 1 (OsHV-1) infection in Pacific oyster Crassostrea gigas spat ArchiMer
Normand, Julien; Blin, Jean-louis; Jouaux, Aude.
Early detection of Pacific oyster spat infected with ostreid herpesvirus 1 (OsHV-1) could prevent introduction of OsHV-1-infected individuals into farming areas or onshore rearing facilities, thus reducing the risk of infection of naïve oysters in such production systems. Experiments were conducted on several hundred oyster spat provided by producers in order to examine whether early rearing practices could be considered as potential risk factors for (1) OsHV-1 infection as detected by molecular methods and (2) spat mortality experimentally induced through thermal challenge. Spat groups collected on oyster beds and hatchery spat reared in growout areas during summer exhibited higher viral DNA contamination and mortalities during the trial than spat kept in...
Tipo: Text Palavras-chave: Oyster spat; Epidemiology; OsHV-1; QPCR; Thermal challenge.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00203/31407/29797.pdf
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Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone ArchiMer
Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf
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An updated assessment of Symbiodinium spp. that associate with common scleractinian corals from Moorea (French Polynesia) reveals high diversity among background symbionts and a novel finding of clade B ArchiMer
Rouze, Heloise; Lecellier, Gael J.; Saulnier, Denis; Planes, Serge; Gueguen, Yannick; Wirshing, Herman H.; Berteaux-lecellier, Veronique.
The adaptative bleaching hypothesis (ABH) states that, depending on the symbiotic flexibility of coral hosts (i.e., the ability of corals to “switch” or “shuffle” their algal symbionts), coral bleaching can lead to a change in the composition of their associated Symbiodinium community and, thus, contribute to the coral’s overall survival. In order to determine the flexibility of corals, molecular tools are required to provide accurate species delineations and to detect low levels of coral-associated Symbiodinium. Here, we used highly sensitive quantitative (real-time) PCR (qPCR) technology to analyse five common coral species from Moorea (French Polynesia), previously screened using only traditional molecular methods, to assess the presence of...
Tipo: Text Palavras-chave: Corals; French polynesia; Clade B; Symbiodinium; QPCR; Flexibility; Generalist; Faithful clade.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00365/47652/47683.pdf
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First comparison of French and Australian OsHV-1 mu vars by bath exposure ArchiMer
Burge, Colleen A.; Reecez, Kimberly S.; Dhar, Arun K.; Kirkland, Peter; Morga, Benjamin; Degremont, Lionel; Faury, Nicole; Wippel, Bryanda J. T.; Macintyre, Alanna; Friedman, Carolyn S..
Economically devastating mortality events of farmed and wild shellfish due to infectious disease have been reported globally. Currently, one of the most significant disease threats to Pacific oyster Crassostrea gigas culture is the ostreid herpesvirus 1 (OsHV-1), in particular the emerging OsHV-1 microvariant genotypes. OsHV-1 microvariants (OsHV-1 mu vars) are spreading globally, and concern is high among growers in areas unaffected by OsHV-1. No study to date has compared the relative virulence among variants. We provide the first challenge study comparing survival of naive juvenile Pacific oysters exposed to OsHV-1 mu vars from Australia (AUS mu var) and France (FRA mu var). Oysters challenged with OsHV-1 mu vars had low survival (2.5%a exposed to AUS...
Tipo: Text Palavras-chave: Ostreid herpesvirus 1; Microvariant; OsHV-1 mu vars; POMS; Pacific oyster; Crassostrea gigas; Viral disease; QPCR; Alkalinity; Emerging infectious diseases.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00631/74277/73889.pdf
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Signature d'expression de gènes, Marqueur prédictif de capacités de survie chez l'huître Crassostrea gigas ArchiMer
Bachere, Evelyne; Degremont, Lionel; Piquemal, David; Roman, Bruno; Noguier, Florian.
Dans le contexte des mortalités massives d'huîtres Crassostrea gigas, les mécanismes moléculaires et les bases génétiques impliquées dans la capacité de survie des huîtres ont été explorées par des approches de génomique à très haut débit. Ces travaux se sont centrés dans un premier temps sur la survie à des infections par des Vibrio virulents pour lesquels des infections expérimentales étaient reproductibles et standardisées en laboratoire. Ces analyses ont conduit à la mise en évidence d'une série de gènes dont l’expression peut prédire la capacité des huîtres à survivre aux infections à vibrios (Rosa et al. 2012). Depuis 2013, les travaux ont permis la validation de la signature de survie qui a pu être corrélée avec les capacités de survie des huîtres...
Tipo: Text Palavras-chave: Huître creuse; Signature d'expression de gènes; QPCR; Biostatistique; Capacité de survie; Phénotypage; Héritabilité; Pacific oyster; Gene expression signature; QPCR; Biostatistics; Survival capacity; Phenotyping; Heritability.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00434/54551/55925.pdf
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Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures Rev. Bras. Ciênc. Avic.
Moraes,DMC; Duarte,SC; Bastos,TSA; Rezende,CLG; Leandro,NSM; Café,MB; Stringhini,JH; Andrade,MA.
Abstract Conventional bacteriology techniques and quantitative polymerase-chain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: QPCR; Eggshells; Albumen; Yolk.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100117
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Effects of in Ovo Infusion of Probiotic Strains on Performance Parameters, Jejunal Bacterial Population and Mucin Gene Expression in Broiler Chicken Rev. Bras. Ciênc. Avic.
Majidi-Mosleh,A; Sadeghi,AA; Mousavi,SN; Chamani,M; Zarei,A.
ABSTRACT The objective of the present study was to evaluate the effects of in ovo infusion of probiotic strains (Bacillus subtilis, Enterococcus faecium, and Pediococcus acidilactici ) on jejunal microbial population and mucin gene expression in broiler chicken. In a completely randomized design, 0.5 ml of mediums containing 107 cfu of different probiotic strains, was administered into amniotic fluid of the 480 Cobb fetus (day 18 of incubation), with four treatments, five replicates with twenty four eggs each. For mucin gene expression, samples from the jejunum were taken on day 21 of incubation and day 3 post-hatch. Microbial profile was determined for total lactobacillus and E. coli by sampling jejunal contents on days 1 and 3 of age. Expression of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In ovo infusion; Jejunum; Microbial population; QPCR; Probiotic.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500097
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Relative expression of CYP and Arabinogalactan genes in soybean seed coats Rev. Ciênc. Agron.
Bahry,Carlos André; Venske,Eduardo; Perboni,Anelise Tessari; Zimmer,Paulo Dejalma.
ABSTRACT The aim of this study was to evaluate the relative expression of two candidate genes, CYP (cyclophilin) and Arabinogalactan, possibly involved with seed quality, in contrasting soybean seed coats. The following genotypes were used: BMX Potência RR and CD 202, both with yellow coats, and TP and IAC, with black coats. The relative expression of the genes was evaluated by qPCR in seven development phases of the coats of the four genotypes at 25; 30; 35; 40; 45; 50 and 55 days after anthesis. The design was completely randomized, with three replicates. The data were submitted to analysis of variance and the means were compared via the Scott Knott test, at 5% probability. The CYP gene showed greater expression in the intermediate stages of development...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Contrasting genotypes; Candidate genes; Cyclophilin; QPCR.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000100078
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Cuantificación de maíz genéticamente modificado mediante las técnicas de qPCR y dPCR Agrociencia
Gutiérrez-Angoa,Lizbeth E.; Castillo-Durán,Luis C.; Gómez-Castelo,Blanca E.; Acatzi-Silva,Abraham I..
A partir de la emisión de los permisos de liberación al ambiente de maíz (Zea mays L.) genéticamente modificado (GM) en el año 2009, en México fue necesario realizar la detección, identificación y cuantificación de organismos genéticamente modificados (OGM) de los cultivos en el país. El objetivo del presente estudio fue validar la técnica de cuantificación absoluta de mezclas de hoja en fracción masa de maíz GM a través de la técnica de la reacción en cadena de la polimerasa (PCR) en tiempo real (qPCR) y digital (dPCR). Mezclas de hojas de maíz modificado con el evento MON810 se prepararon con hojas de maíz convencional, fueron analizadas con la técnica de qPCR, y como calibrantes se usaron un plásmido de referencia certificado y ADN obtenido de una hoja...
Tipo: Info:eu-repo/semantics/article Palavras-chave: QPCR; DPCR; Zea mays L.; OGM.
Ano: 2015 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000400003
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Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools Anais da ABC (AABC)
STAGGEMEIER,RODRIGO; ARANTES,THALITA; CAUMO,KARIN S.; ROTT,MARILISE B.; SPILKI,FERNANDO R..
ABSTRACT Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas isolated from water samples collected from swimming pools in the city of Porto Alegre, Southern Brazil. Free-living amoebae of the genus Acanthamoeba were isolated from water samples, and isolates (n=16) were used to investigate the occurrence of HAdVs. HAdV detection was performed by quantitative real-time polymerase chain reaction (qPCR). HAdVs were detected in 62.5% (10/16) of Acanthamoeba isolates, ranging from 3.24x103 to 5.14x105 DNA copies per milliliter...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acanthamoeba; Free-living amoebae; Human adenovirus; QPCR; Swimming pools.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200635
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Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient Arq. Bras. Med. Vet. Zootec.
Resende,M.V.; Lucio,A.C.; Perini,A.P.; Oliveira,L.Z.; Almeida,A.O.; Alves,B.C.A.; Moreira-Filho,C.A.; Santos,I.W.; Hossepian de Lima,V.F.M..
The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR) of sperm DNA and resultant in vitro-produced bovine embryos by PCR. Frozen/thawed sperm was layered on density gradients and the tubes were centrifuged. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Cleavage and blastocyst rates were determined through in vitro production of embryos and PCR was performed to identify the embryos' genetic sex. A difference in blastocyst rate was found in the Percoll treatment compared to OptiPrep (P<0.05). The percentage of female embryos in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Sperm sexing; Centrifugation; Embryo sexing; QPCR; PCR.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300002
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Occurrence of Mycobacterium avium subsp. paratuberculosis in coalho cheese in the State of Pernambuco, Brazil Arq. Bras. Med. Vet. Zootec.
Albuquerque,P.P.F.; Cezar,R.D.S.; Pinheiro Junior,J.W.; Grazielle Nascimento,G.; Santos,A.S.; Mota,R.A..
ABSTRACT Paratuberculosis is a chronic and incurable disease that affects ruminants and other domestic animals. It is caused by Mycobacterium avium subsp. paratuberculosis (MAP) that may also be involved in some human diseases such as Crohn's disease, type 1 diabetes, sarcoidosis, multiple sclerosis, and Hashimoto's thyroiditis. The objective of this study was to investigate the occurrence of MAP DNA in samples of artisanal coalho cheese purchased in the State of Pernambuco. Forty samples of coalho cheese submitted to the Real Time Polymerase Chain Reaction (qPCR) technique were analyzed for the detection of the MAP region IS900. 11 (27.5%) were positive with a mean of 195.9 MAP colony forming unit (CFU) per gram of each sample, with a minimum of 30.3...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; IS900; QPCR; Paratuberculosis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601917
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Molecular investigation of Neorickettsia risticii in trematodes and snails in a region with serological evidence of this agent in horses, state of Rio de Janeiro Arq. Bras. Med. Vet. Zootec.
Costa,R.L.; Vitari,G.L.V.; Silva,C.B.; Peckle,M.P.; Pires,M.S.; Brandolini,S.V.P.B.; Pinheiro,J.; Massard,C.L.; Santos,H.A..
ABSTRACT In Brazil, some studies have indicated that Neorickettsia risticii circulates in horses, but it is unclear which are the possible intermediate vectors of this bacterium in the country. The aim of this study was to use molecular techniques in order to analyze the presence of N. risticii in snails and larval stages of trematodes in farms in a region with a history of seroreactive horses towards this bacterium, in Rio de Janeiro, Brazil. Convenience sampling was used in the studied region. The collected snails were exposed to incandescent light (60W) for 2-4 hours in order to investigate trematodes in larval forms. Deoxyribonucleic acid (DNA) was extracted from snail tissue and trematode. Real-time PCR (qPCR) technique was used to investigate the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine neorickettsiosis; Molecular diagnosis; QPCR; Intermediate hosts.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601470
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